[RESEARCH] [PUBLICATIONS]

 

Dr. Amro Zayed
Assistant Professor
Department of Biology
York University
4700 Keele Street
Toronto, ON M3J 1P3 Canada
Email: zayed[at]yorku.ca

 

NEW - The Amro Zayed Lab @ York U.

I am pleased to announce that I will be starting a faculty position at York University’s Department of Biology (Toronto, Canada) during the summer of 2009, where I will continue my research on the evolutionary genomics of bees. My laboratory will provide graduate students and postdoctoral fellows with an integrative environment to conduct research at the intersection of ecology, evolution, and genomics, utilizing the latest technology (e.g. microarrays, SNPs, next-generation sequencing, etc.). Funding is available for several graduate students. If you are interested in joining my laboratory, please email me your CV and a statement of research interests.

Please visit my faculty webpage at York U for the latest research news.

Sincerely, Amro

Academic Background:
Ph.D. Biology, York University
(Toronto, Canada)

Research Interests: I am broadly interested in how genetic diversity - diversity at the lowest form of biological organization - affects individual, population and community level traits at both ecological and evolutionary time scales. I prefer to take an interdisciplinary approach to research by integrating theoretical and modeling exercises with empirical and experimental (when possible) studies, utilizing population genetics, molecular biology, genomics, and ecological methods.

Postdoctoral Research: I am currently conducting research in Dr. Charlie Whitfield's lab at the University of Illinois at Urbana-Champaign. We are attempting to study the genetic basis of division of labor in Honeybee colonies using a 'systems' approach combining quantitative genetics (quantitative trait mapping) and genomics (to measure global gene expression - the first phenotype!). Division of labor, or 'task' specialization, is an important feature of insect colonies, and likely at the root of their ecological dominance. In the honeybee, workers undergo an age-related shift in behavior, from in-hive tasks such as nursing to outside tasks such as collecting pollen and nectar. We hope to show how genetic variation influences a complex trait such as division of labor through intermediate phenotypes (such as gene expression, physiology).

In the meantime, Dr. Whitfield and I recently published a paper showing that positive selection has facilitated adaptation in natural and invasive honey bee populations [article, press release, USA Today article, Inside Illinois Story, John Hawks' blog].

Doctoral Research: My Ph.D. dissertation, supervised by Dr. Laurence Packer, focused on bee population and conservation genetics. Why bees? They are the principal pollinators in almost all terrestrial ecosystems, and they play a major role in agricultural systems as well... Bees pollinate about 70% of the crops that feed the world, generating billions of dollars annually !!!... Pollination has been highlighted as a degraded ecosystem service in the latest Millennium Ecosystem Assessment: declines in pollinator populations have been reported worldwide. We need to research methods of protecting and conserving bee populations.

My thesis mainly involved how sex determination affects the population biology of bees, ants and wasps. In bees, a strange and unique phenomenon can sometimes occur due to complementary sex determination - the production of diploid males. Male bees are usually haploid, but under certain circumstances (involving inbreeding or loss of allelic diversity in small populations) diploid males are produced instead of females (see Figure 1). Diploid males do not reproduce or help around in the nest, so their production is highly disadvantageous. Through a modeling study, we discovered that the production of diploid males GREATLY increases the extinction risk in small bee populations (see article , also see Toronto Star article, TVO's interview, Radio Canada interview, press release, Y-File, NSERC Story, Research Focus feature in Trends in Ecology and Evolution by P. Hedrick, J. Gadau and R.E. Page Jr.). Further, my colleagues and I have proposed the use of diploid male production as a parameter to indicate pollinator decline (see article, also see press release, PRSL Summary, feature on Y-File, York U article). I also plan on examining the effects of diploid male production on bee metapopulaion dynamics, both theoretically and empirically.

I also examined the effects of pollen specialization on the population genetics of oligolectic bees. Oligolectic, or specialist bees collect pollen from only a specific flower taxon. Polylectic bees, on the other hand, collect pollen from many taxa. Oligolectic bees might be expected to persist in smaller and more isolated populations than polylectic bees. We have recently found evidence supporting this hypothesis (see article, also see Excalibur story). In a study of specialist and generalist bees in Chile, we found that specialist bees have lower levels of genetic diversity when compared to closely related generalists, which is indicative of specialists persisting in smaller effective populations. We also found higher levels of genetic differentiation within populations of a specialist bee when compared to a generalist, implying that specialists live in more isolated populations (see article). Significant population structure has also been found in a North American evening primrose specialist (Zayed and Packer, 2007) using newly developed microsatellite markers for the bee genus Lasioglossum (Zayed 2006).

If you have any questions and comments on my research, please contact me. Thanks for visiting my site.

Amro Zayed, Ph.D.
zayed[at]yorku.ca

Publications:

12. Zayed, A., Whitfield, C.W. (2008). A genome-wide signature of positive selection in ancient and recent invasive expansions of the honey bee Apis mellifera. Proceedings of the National Academy of Sciences USA, 105:3421-3426. [article, press release, USA Today article, Inside Illinois Story, John Hawks' blog]

11. Zayed, A., Constantin, S.A. and Packer, L. (2007) Successful biological invasion despite a severe genetic load. PLoS ONE 2:e868. doi:10.1371/journal.pone.0000868. [article, press release, Conservation Magazine story]

10. Zayed, A., and Packer L. 2007. Population genetics of a solitary oligolectic sweat bee, Lasioglossum (Sphecodogastra) oenotherae (Hymenoptera: Halictidae). Heredity, 99: 397-405. [pdf]

9. Zayed, A. 2006. Characterization of microsatellite loci from the solitary sweat bees Lasioglossum leucozonium and Lasioglossum oenotherae (Hymenoptera, Halictidae). Molecular Ecology Notes, 6: 1154-1156. [pdf]

8. Zayed, A., and Packer, L. 2005. Complementary sex determination substantially increases extinction proneness of haplodiploid populations. Proceedings of the National Academy of Sciences USA, 102:10742-10746.[pdf, Toronto Star article, TVO's interview, Radio Canada interview, press release, Y-File, NSERC Story, Research Focus feature in Trends in Ecology and Evolution by P. Hedrick, J. Gadau and RE Page Jr.]

7. Zayed, A., Packer, L., Grixti, J.C., Ruz, L., Toro, H., and Owen, R. 2005. Increased genetic differentiation in a specialist versus a generalist bee: implications for conservation. Conservation Genetics, 5:1017-1026. [pdf]

6. Packer, L.*, Zayed, A.*, Grixti, J.C., Ruz, L., Owen, R., Vivallo, F., and Toro, H. 2005. Conservation genetics of potentially endangered mutualisms: reduced levels of genetic variation in specialist versus generalist bees. Conservation Biology. 19:195-202. [pdf, Excalibur story]
* L. Packer and A. Zayed contributed equally to this paper.

5. Zayed, A. 2004. Effective population size in Hymenoptera with complementary sex determination. Heredity, 93:627-630. [pdf]

4. Zayed, A., Roubik, D.W., And Packer, L. 2004. Use of diploid male frequency data as an indicator of pollinator decline. Proceedings of the Royal Society of London B. 271, S9-S12. [pdf, press release, PRSL Summary, feature on Y-File, York U article]

3. Grixti, J.C., Zayed, A., and Packer, L. 2004. Behavioral interactions among females of Acamptopoeum submetallicum (Spinola) and Nolanomelissa toroi Rozen (Hymenoptera: Andrenidae). Journal of Hymenoptera Research. 13:48-56.

2. Zayed, A. and Packer, L. 2002. Genetic differentiation across a behavioural boundary in a primitively eusocial bee, Halictus poeyi Lepeletier (Hymenoptera: Halictidae). Insectes Sociaux, 49:282-288. [pdf]

1. Zayed, A. and Packer, L. 2001. High levels of diploid male production in a primitively eusocial bee (Hymenoptera: Halictidae). Heredity, 87:631-636. [pdf]